DBT HEALTHCARE MOLECULAR BIOLOGY/VIROLOGY LABORATORY

PRINCIPAL INVESTIGATOR: DR. LAHARI SAIKIA
Professor & Head, Department of Microbiology, Assam Medical College & Hospital, Dibrugarh
Co PI: Dr. Jayanta Kr. Das
Associate Professor, Department of Microbiology, Assam Medical College & Hospital, Dibrugarh
Date of initiation: 24th September 2009


Number and categories of personnel in the laboratory

The following personnel are employed on a contractual basis for the  laboratory as per the DBT guidelines:

  1. Junior Research Fellow: one
  2. Data Entry Operator: one
  3. Laboratory technicians: Two
  4. Laboratory Attendant: One
  5. Cleaner: One
Major Equipments and Facility Available
 
  Capillary DNA Sequencer
 
  Cobas TaqMan 48 (RT-PCR) Conventional PCR
 
  Gel Documentation system Refrigerated plate Centrifuge
 
  Bio Safety Cabinet   -80° C Deep Freezer
 
  Fluorescent inverted Microscope CO2 Incubator

DBT Molecular Biology/ virology laboratory occupying a area of 522 sq. feet is well equipped with separate pre PCR and Post PCR rooms, Bio-safety cabinets, Deep Freezers, Table top and Floor top refrigerated centrifuge, conventional PCR, Real Time PCR, Gel Documentation System, Fluorescent Inverted Microscope, Hot Air Oven, CO2 Incubator, 8 Capillary DNA Sequencer. A well equipped Tissue culture laboratory has also been established under DBT to carry out various research work such as maintenance of cell line, cryopreservation, isolation and identification of viruses.


Diagnostic facilities available and Tests offered for Patient service
  1. HBV VIRAL LOAD (Hepatitis B DNA Quantitative): Test Done in ROCHE Cobas TaqMan 48 Real Time PCR
  2. HIV VIRAL LOAD (HIV RNA Quantitative): Test Done in ROCHE Cobas TaqMan 48 Real Time PCR
  3. Multi Drug Resistance (MDR) assay in MTB positive cases : Test done in HAIN Twin Incubator
Ongoing Research Activity and Sevice
  1. Research work going on under CDC project “Strengthening laboratory support for surveillance to JE in India’’, with NIMHANS for detection bacterial and viral agents other than JE.
  2. DNA Sequencing service is being offered by Molecular Biology Lab.
  3. The existing facilities of Molecular Biology, Virology Lab is being utilized to carry out MD thesis work and Extra Mural Research project works.